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KMID : 0545120050150020376
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Journal of Microbiology and Biotechnology
2005 Volume.15 No. 2 p.376 ~ p.383
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Analysis of Plasmid pJP4 Horizontal Transfer and Its Impact on Bacterial Community Structure in Natural Soil
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Kim TS
Kim MS/Jung MK/Jeo MJ/Ahn JH/Oh KH/Lee MH/Kim MK/Ka JO
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Abstract
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Alcaligenes sp. JMP228 carrying 24- dichlorophenoxyacetic acid (24-D) degradative plasmid pJP4 was inoculated into natural soil and transfer of the plasmid pJP4 to indigenous soil bacteria was investigated with and without 24-D amendment. Plasmid pJP4 transfer was enhanced in the soils treated with 24-D compared to the soils not amended with 24-D. Several different transconjugants were isolated from the soils treated with 24-D while no indigenous transconjugants were obtained from the unamended soils. Inoculation of the soils with both the donor Alcaligenes sp. JMP228/pJP4 and a recipient Burkholderia cepacia DBO1 produced less diverse transconjugants than the soils inoculated with the donor alone. Repetitive extragenic palindromic-polymerase chain reaction (REP-PCR) analysis of the transconjugants exhibited seven distinct genomic DNA fingerprints. Analysis of 16S rDNA sequences indicated that the transconjugants were related to members of the genera Burkholderia and Pandoraea. Denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rRNA genes revealed that inoculation of the donor caused clear changes in the bacterial community structure of the 24-Damended soils. The new 16S rRNA gene bands in the DGGE profile corresponded with the 16S rRNA genes of 24-Ddegrading transconjugants isolated from the soil. The results indicate that introduction of the 24-D degradative plasmid as Alcaligenes sp. JMP228/pJP4 has a substantial impact on the bacterial community structure in the 24-D-amended soil.
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